Common types of containers used to grow cells include t-flasks, roller bottles, cell factories, and spinner flasks. What determines the container used is the type of cells you are attempting to expand and/or size of the culture. The two prominent types of cell shape and growth style are adherent cells and suspension cells.
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Adherent cells need a surface to which they can attach to for growth and multiplication. T-flasks, roller bottles and cell factories can be made with coated surfaces to which adherent cells may attach themselves. Surface area is the measurement criteria in these cases.
On the other hand, suspension cells are able to grow “suspended” or free-floating in a liquid growth medium. Spinner flasks are commonly chosen based on desired quantity of cells and use volume measurements, such as liters and milliliters.
One coated side of a T-flask for adherent cells is used for cells to grow upon. A suspension cell line would use a non-coated T-flask. These are ideal when you are beginning from a small quantity of cells, such as the start of a cell culture from a single thawed cell bank vial.
Roller bottles are slowly rotated with a machine, which allows the cells to grow on the whole inner surface of the container. As the container rotates, exposure occurs between the liquid media and the cells inside of the container. When higher quantities of cells are required, roller bottles are often the desirable choice as cells can grow on all of the inner surface. Often a cell culture is started in a T-flask with roller bottles serving as the next step up as cell quantities increase. Similarly to T-flasks, adherent cells would require a coated bottle and suspension cells would require a non-coated bottle.
Cell culture amplification utilizes cell factories for industrial production of monoclonal antibodies and vaccines or in a reduced size lab space. In addition, this smaller footprint will allow for reduced manipulation, less labor hours needed for maintenance, and lower equipment requirements of a lab space.
These flasks utilize a rotating magnet plate, driving a magnetic stirrer. This stirrer provides uniform, constant agitation, preventing the cells from settling to the bottom and keeping them uniformly distributed throughout the growth medium. This vessel is only appropriate for use with suspension cell lines. Hybridomas can be grown in spinner flasks for monoclonal antibody production, in which the antibody-enriched supernatant is purified. At Cell Culture Company, we are your source for various cell and antibody production services.
To learn about the services we offer for cell culture growth and multiplication give us a call today at 763.786.0302 or reach us through our contact form.
Roller bottles are widely used in cell culture for a variety of applications, offering several advantages over traditional cell culture vessels. Roller bottle systems consist of cylindrical plastic bottles that are gently rotated or rocked on a roller apparatus. In this response, I will outline the benefits and applications of roller bottle cell culture.
Large Surface Area: Roller bottles provide a relatively large surface area for cell growth compared to traditional culture dishes or flasks. The cylindrical shape of roller bottles allows for a larger culture area per unit volume, maximizing the available surface area for cell attachment and proliferation. This increased surface area facilitates the growth of a larger number of cells and can be beneficial for scaling up cell production.
Enhanced Oxygen and Nutrient Exchange: The rolling motion of the roller bottles improves oxygen and nutrient exchange in the culture medium. The gentle rotation promotes the mixing and circulation of the medium, ensuring a more homogeneous distribution of oxygen and nutrients throughout the culture. This enhanced oxygenation and nutrient supply support optimal cell growth and metabolism.
Cell Culture Roller Bottle
Reduced Shear Stress: Roller bottle systems typically operate at lower speeds, generating less shear stress on the cells compared to more vigorous agitation methods, such as stirred bioreactors or spinner flasks. This lower shear stress minimizes the potential for cell damage or detachment during culture, particularly for delicate or sensitive cell types.
Scalability: Roller bottles are scalable, allowing for easy expansion of cell cultures. Multiple roller bottles can be stacked and simultaneously rotated on a roller apparatus, enabling increased culture volume without significantly increasing the required space or equipment. This scalability is particularly advantageous for large-scale production of cells, viruses, or other cellular products.
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Easy Sampling and Manipulation: Roller bottles provide easy access to the culture medium, allowing for convenient sampling and manipulation of the cells. The culture medium can be aseptically sampled or supplemented with nutrients, growth factors, or other additives without disturbing the cells or compromising sterility. This accessibility simplifies experimental procedures, media changes, and the addition of specific reagents or treatments.
Vaccine Production: Roller bottles are widely used in the production of viral vaccines, such as influenza, polio, or measles vaccines. The large surface area and scalability of roller bottle systems facilitate the growth of large quantities of virus-infected cells, ensuring sufficient viral yield for vaccine production.
Cell Line Development: Roller bottle systems are commonly employed for establishing and maintaining stable cell lines for research or industrial purposes. The large surface area and gentle rolling motion provide an optimal environment for the attachment, growth, and expansion of anchorage-dependent cell lines.
Expanded Surface Polystyrene Roller Bottle
Virus Production: Many viruses require anchorage-dependent cells for efficient replication. Roller bottle culture systems support the growth of these cells, making them suitable for virus propagation and production. The enhanced oxygen and nutrient exchange in roller bottles help maintain high cell viability and support robust viral replication.
Antibody Production: Roller bottle cultures are used for large-scale production of monoclonal antibodies and other recombinant proteins. The increased surface area and scalability of roller bottle systems enable the growth of high-density cultures, resulting in higher antibody yields.
Tissue Engineering: Roller bottles are also employed in tissue engineering research and applications. They provide a suitable environment for the growth and differentiation of stem cells, facilitating the formation of tissue-like structures. The scalability of roller bottle systems allows for the generation of larger tissue constructs for transplantation or in vitro studies.
Research and Screening: Roller bottles are utilized in various research applications, including screening studies, drug discovery, and cytotoxicity testing. The large surface area and easy manipulation of roller bottle cultures allow for the evaluation of cell responses to specific compounds, drugs, or environmental factors.
In summary, roller bottle cell culture systems offer several advantages, including a large surface area, enhanced oxygen and nutrient exchange, reduced shear stress, scalability, and ease of manipulation. These features make roller bottles suitable for a range of applications, such as vaccine production, cell line development, virus propagation, antibody production, tissue engineering, and research studies. Roller bottle systems provide an efficient and flexible platform for growing and expanding cells in a controlled and reproducible manner, supporting various cellular and molecular biology research, as well as industrial applications.
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